Infections caused by pathogenic bacteria such as Gram-positive Staphylococcus aureus and Gram -negative Pseudomonas aeruginosa remain a significant healthcare challenge. In this study, we devel-oped an enzyme-responsive antibacterial nanomaterial that delivers antibacterial agents only in the presence of bacterial lipase. This was achieved by first synthesizing highly potent ultra-small silver nanoparticles with an average core size of 1.6 & PLUSMN; 0.2 nm and then embedding them into polycaprolactone nanoparticles (pAgNCs@PCL) via the water in oil in water approach. The pAgNCs@PCL nanoparticles had an average diameter of 274.1 & PLUSMN; 60.1 nm and a silver nanoparticle encapsulation efficiency of 15.7 & PLUSMN; 1.3%. We demonstrated that silver released from the pAgNCs@PCL was selectively triggered by lipase-expressing strains of P. aeruginosa and S. aureus. As expected, there was no antibacterial action observed against E. coli DH5a, which does not express lipase. The pAgNCs@PCL nanoparticles exhibited minimal cytotoxicity against human fibroblast cells suggesting good biocompatibility. This study resulted in a highly potent on-demand delivery system that can be potentially incorporated into healthcare products and devices to prevent and/or treat infections.& COPY; 2023 Elsevier Ltd. All rights reserved.
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