Abstract:
Hexavalent chromium-tolerant (1500mg/L) bacterium MW1 was isolated from harbour water of Paradip Port and evaluated for Cr(VI) reduction potential. The isolate was identified as Exiguobacterium indicum by biochemical and 16S rRNA gene sequence methods. Salt tolerance of the bacterium was evaluated in a wide range of NaCl concentrations (0.5-13%, w/v). The Cr(VI) reduction of the strain was evaluated and optimised with varied Cr(VI) concentrations (100-1000mg/L), pH (5.0-9.0), temperature (30-40 degrees C) and shaking velocity (100-150rpm) in two different minimal media (M9 and Acetate). Under optimised conditions, after 192h of incubation nearly 92%, 50% and 46% reduction in the M9 minimal medium and 91%, 47% and 40% reduction in the acetate minimal medium were observed for 100, 500 and 1000mg/L of Cr(VI), respectively. The exponential rate equation for Cr(VI) reduction yielded higher rate constant value, that is, 1.27x10(-2)h(-1) (M9) and 1.17x10(-2)h(-1) (Acetate) in case of 100mg/L and became lower for 500 and 1000mg/L Cr(VI) concentrations. Further, the association of bacterial cells with reduced product was ascertained by Fourier transform infrared spectrometer, UV-Vis-DRS and field-emission scanning electron microscope-energy-dispersive X-ray analyses. The above study suggests that the higher reducing ability of the marine bacterium E. indicum MW1 will be suitable for Cr(VI) reduction from saline effluents.