Multiple shoots were induced on hypocotyl segments reared from in vitro germinated seedlings of Jatropha curcas L., using Murashige and Skoog (MS) medium supplemented with N6-benzyl adenine (BA) and kinetin (kin) either alone or in combination with indole-3-acetic acid (IAA). The combined treatment of 7.05 mu m kin and 1.425 mu m IAA resulted in maximum shoot production with an average shoot bud initiation of 12.1 per explant. The regenerated microshoots were transferred to root induction medium containing half-strength MS salts supplemented with either indole-3-butyric acid (IBA) or IAA. Rooting of microshoots was best achieved on half-strength MS medium supplemented with IBA (9.8 mu m). Rooted plantlets were subsequently acclimatized under green house condition and the plantlets showed 70% survival.
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