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Characterization of urethanase from Micrococcus species associated with the marine sponge (Spirastrella species)

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dc.contributor.author Mohapatra, B.R.
dc.contributor.author Bapuji, M.
dc.date.accessioned 2018-10-01T12:20:17Z
dc.date.available 2018-10-01T12:20:17Z
dc.date.issued 1997
dc.identifier.citation Letters In Applied Microbiology, 25(6), 1997: 393-396
dc.identifier.issn 0266-8254
dc.identifier.uri http://ore.immt.res.in/handle/2018/626
dc.description.abstract Eighty per cent of the urethanase activity of Micrococczu sp. isolated from the marine sponge Spirastrella sp. was cell associated. The urethanase had optimal activity at pH 5.0 and 45 degrees C. The activation and deactivation energies of the partially purified enzyme, calculated from an Arrhenius plot, were 9.31 and 34.01 kcal mol(-1), respectively. The enzyme was not affected by EDTA and the major cations of sea water, such as Ca2+, Mg2+ and Na+. The enzyme was resistant to 20% (v/v) ethanol and may be practically applicable in the removal of urethane from alcoholic beverages.
dc.language en
dc.publisher Blackwell
dc.relation.isreferencedby SCI
dc.rights Copyright [1997]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subject Biological Sciences
dc.subject Biological Sciences
dc.title Characterization of urethanase from Micrococcus species associated with the marine sponge (Spirastrella species)
dc.type Journal Article
dc.affiliation.author CSIR-IMMT, Bhubaneswar 751013, Odisha, India


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