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Minimum number of oligonucleotide-based stable monomeric branched DNA nanostructure: Biochemical and biophysical study

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dc.contributor.author Kar, A
dc.contributor.author Baral, B
dc.contributor.author Subudhi, U
dc.date.accessioned 2025-07-22T08:55:15Z
dc.date.available 2025-07-22T08:55:15Z
dc.date.issued 2024
dc.identifier.citation International Journal Of Biological Macromolecules, 276, 2024; 133930
dc.identifier.issn 0141-8130
dc.identifier.uri http://ore.immt.res.in/handle/2018/3590
dc.description CSIR-Young Scientist Project [YSP-05]; Har Gobind Khorana DBT-IYBA project [GAP-312]; Indian Council of Medical Research, New Delhi
dc.description.abstract DNA has been employed as building blocks for the construction of nanomaterials due to their programmability and wide range applications. The functional branched DNA (bDNA) nanostructure is largely dependent on the sequence and structural symmetry. Despite the discovery of different structures, the synthesis of bDNA nano- structures from optimal number of oligonucleotides is yet to be explored. In the current study, for the first time we demonstrate the designing of stable monomeric bDNA structures using two or three oligonucleotides. Furthermore, the stability of bDNA nanostructures was thoroughly investigated in presence of different pH, cations, fetal bovine serum and DNase I. The thermodynamic parameters indicated that hydrogen bonding and van der Waals interactions played a major role during self-assembly of bDNA nanostructures. From the gel retardation assay, we confirmed the binding of complementary oligonucleotides to the bDNA nanostructures, thus can be explored for target specific transcript regulation. In conclusion, the self-assembled DNA nano- structures developed from optimal oligonucleotides are stable in physiological environment and can be used for biomedical applications.
dc.language en
dc.publisher Elsevier
dc.relation.isreferencedby SCI
dc.rights Copyright [2024]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subject Biochemistry & Molecular Biology
dc.subject Chemical Sciences
dc.subject Polymer Science
dc.title Minimum number of oligonucleotide-based stable monomeric branched DNA nanostructure: Biochemical and biophysical study
dc.type Journal Article
dc.affiliation.author CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751013, Odisha, India


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