dc.contributor.author |
Kar, A |
|
dc.contributor.author |
Baral, B |
|
dc.contributor.author |
Subudhi, U |
|
dc.date.accessioned |
2025-07-22T08:55:15Z |
|
dc.date.available |
2025-07-22T08:55:15Z |
|
dc.date.issued |
2024 |
|
dc.identifier.citation |
International Journal Of Biological Macromolecules, 276, 2024; 133930 |
|
dc.identifier.issn |
0141-8130 |
|
dc.identifier.uri |
http://ore.immt.res.in/handle/2018/3590 |
|
dc.description |
CSIR-Young Scientist Project [YSP-05]; Har Gobind Khorana DBT-IYBA project [GAP-312]; Indian Council of Medical Research, New Delhi |
|
dc.description.abstract |
DNA has been employed as building blocks for the construction of nanomaterials due to their programmability and wide range applications. The functional branched DNA (bDNA) nanostructure is largely dependent on the sequence and structural symmetry. Despite the discovery of different structures, the synthesis of bDNA nano- structures from optimal number of oligonucleotides is yet to be explored. In the current study, for the first time we demonstrate the designing of stable monomeric bDNA structures using two or three oligonucleotides. Furthermore, the stability of bDNA nanostructures was thoroughly investigated in presence of different pH, cations, fetal bovine serum and DNase I. The thermodynamic parameters indicated that hydrogen bonding and van der Waals interactions played a major role during self-assembly of bDNA nanostructures. From the gel retardation assay, we confirmed the binding of complementary oligonucleotides to the bDNA nanostructures, thus can be explored for target specific transcript regulation. In conclusion, the self-assembled DNA nano- structures developed from optimal oligonucleotides are stable in physiological environment and can be used for biomedical applications. |
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dc.language |
en |
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dc.publisher |
Elsevier |
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dc.relation.isreferencedby |
SCI |
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dc.rights |
Copyright [2024]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository. |
|
dc.subject |
Biochemistry & Molecular Biology |
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dc.subject |
Chemical Sciences |
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dc.subject |
Polymer Science |
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dc.title |
Minimum number of oligonucleotide-based stable monomeric branched DNA nanostructure: Biochemical and biophysical study |
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dc.type |
Journal Article |
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dc.affiliation.author |
CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751013, Odisha, India |
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