Green synthesis of silver nanoparticles and its potential effect on phytopathogens

Abstract

Nanotechnology provides environmentally friendly green technique for synthesizing silver nanoparticles using various parts of the plant. Here we used aqueous leaf extract of Symplocos racemosa for synthesis of silver nanoparticles (AgNPs) by adding 1 mM AgNO3 as reducing agent. Silver nanoparticles (AgNPs) from aqueous leaf extract and 1 mM AgNO3 are successfully synthesized here. The characterization of the silver nanoparticles were carried out by UV-visible spectroscopy, XRD (X-Ray Diffraction), FTIR (Fourier Transform Infrared Spectroscopy) and Transmission Electron Microscopy (TEM), FE-SEM (Field Emission Scanning Electron Microscope), EDX (Energy Dispersive X Ray Spectroscopy) and Transmission Electron Microscopy (TEM). The limit of UV-visible spectroscopy at 422 nm supported the reduction AgNO3 to AgNPs. FTIR peaks were observed in the frequencies near 3270, 1640, 1408, 1080 cm(-1) implying that O-H stretch, C-C, -CH3, and C-O-C groups respectively from the extracts, which were capped with silver nanoparticles (AgNPs). Antimicrobial activity of synthesized silver nanoparticles have been studied on Pseudomonas aeruginosa. (C) 2020 Elsevier Ltd. All rights reserved.