High frequency plant regeneration through adventitious multiple shoot organogenesis in epicotyl explants of Indian gooseberry (Emblica officinalis Gaertn)

Abstract

Shoots regenerated adventitiously on epicotyl segments from in vitro seedlings of Emblica officinalis var. 'Kanchan'. Epicotyls derived from 2-week-old aseptic seedlings were most responsive and produced a Maximum number of 303 shoots per explant in Murashige and Skoog (1962) medium (MS) augmented with 8.8 mu M N(6)-benzyladenine (BA) + 1.425 mu M indole-3-acetic acid (IAA). Shoots readily elongated in MS lacking growth regulators and rooted in half-salt-strength MS (1/2 MS) supplemented with indole-3-butyric acid (IBA) or alpha-naphthalene acetic acid (NAA). The highest rooting response was recorded in 1/2 MS containing 14.7 mu M IBA. Plantlets were acclimatized inside the green house and 80% of the plantlets survived on transfer to garden soil. (C) 2009 Elsevier B.V. All rights reserved.